The following enemies can be found in the 2001 builds. However, most of these other maps appear to be test maps. For example, both builds contain a 'Zoo' map showcasing most of the in-game assets, similar to the Zoo map from Duke Nukem 3D. There are also other maps included in the files with each build. Notably, the level titled 'Deja Vu' is a recreation of Hollywood Holocaust, the first level of Duke Nukem 3D. The list of levels differs slightly between the two builds included in the prototype:ġ.1.

In the file 'DukeForever.ini', the names of these levels and segments are found under a section titled 'Engine.MapLocations', where the map names correspond to a 'Location' variable and the segments correspond to a 'Name' variable. Based on the titles of these segments, these were likely intended to function as 'chapters' within a given level. Upon stopping the carousel on any particular map, a dropdown box allows the player to specify a segment of the map to spawn. When selecting a level, map names and accompanying screenshots are presented in a horizontally sliding carousel. Levels in the prototype can be selected from the main menu.

Bombshell was a fan-favorite character from the 1998 E3 trailer who was originally planned to appear as Duke Nukem's sidekick, but she was later scrapped from the project, only to appear much later as the main protagonist in two games of her own: Bombshell (2016) and Ion Fury (2019). Notably, the 2001 prototype only mentions but does not actually feature Bombshell. Duke Nukem Forever 2001 is significantly more developed and closer to the final 2011 release than the 1996 Duke Nukem Forever side-scroller ⁠- which is not even a direct antecessor of this prototype ⁠- or the 1998 build shown in an E3 trailer from that year.

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